Proteomic Signatures in Spermatozoa Reveal the Role of Paternal Factors in Recurrent Pregnancy Loss

Effects of prostaglandin administration 10 days apart on reproductive parameters of cyclic dairy nulliparous goats / Efeitos da aplicação de prostaglandinas intervaladas de 10 dias sobre características reprodutivas de cabras leiteiras nulíparas cíclicas

This study reported the effects of prostaglandin (PGF2a) administration 10 days apart on reproductive parameters of cyclic artificial inseminated (AI) nulliparous Alpine (n=9) and Saanen (n=9) goats. Animals received two doses of 22.5mg PGF2a 10 days apart. After 1st and 2nd PGF2a administrations, estrus was monitored at 12 h intervals, with a buck teaser. Plasma progesterone concentration (ng/mL) was determined from blood sampled on day 0 (1st PGF2a) and the following 5, 10 (2nd PGF2a), 15, 20, 25 and 30 days. After the onset of the second estrus, females were transrectally (5 MHz probe) scanned at 4 hour intervals until at least 8h after ovulation. Pregnancy was checked through transrectal ultrasound on days 20, 25, 30, 35 and 90 after insemination. All parameters studied did not differ between breeds (P>0.05). Estrous response and interval to estrus, respectively, after 1st (78.9% and 50.6±17.2h) and 2nd PGF2a (88.9% and 50.0±14.8h) administration did not differ (P>0.05). Overall animals ovulating (100.0%), interval to ovulation after 2nd PGF2a (64.5±19.5h) and after estrous onset (18.0±9.1h), ovulation rate (1.3±0.5), diameter of ovulatory follicle (8.1±1.1mm) were recorded. Embryo loss occurred before day 30 of pregnancy. Estrus can be efficiently synchronized in nulliparous Alpine and Saanen goats with two doses of prostaglandin 10 days apart.

Relataram-se os efeitos da aplicação de prostaglandina sobre características reprodutivas de cabras leiteiras nulíparas cíclicas. Cabras Alpinas (n=9) e Saanen (n=9) receberam duas doses de 22,5mg PGF2a com 10 dias de intervalo. A progesterona plasmática (ng/mL) foi determinada a partir de amostras de sangue coletadas nos dias 0 (primeira dose), 5, 10 (segunda dose), 15, 20, 25 e 30. Após início do segundo estro, as fêmeas foram monitoradas por ultrassonografia transretal a cada quatro horas até oito horas após a ovulação. A gestação foi verificada por ultrassonografia transretal nos dias 20, 25, 30, 35 e 90 após a segunda dose. As características estudadas foram semelhantes entre as raças (P>0,05). Animais em estro e o intervalo parto-estro de, respectivamente, 78,9% e 50,6±17,2h e 88,9% e 50,0±14,8h após a primeira e segunda administrações de prostaglandina, não diferiram (P>0,05). Todas as cabras ovularam e registraram-se valores do intervalo parto-ovulação após a segunda aplicação de prostaglandina de 64,5±19,5h e após início do estro de 18,0±9,1h, a taxa de ovulação de 1,3±0,5 e diâmetro do folículo ovulatório de 8,1±1,1mm. Perda embrionária ocorreu antes de 30 dias de gestação. O estro pode ser eficientemente sincronizado em cabras leiteiras núliparas com duas doses de prostaglandina intervaladas de 10 dias.

Uso de la heparina de bajo peso molecular y aspirina en una paciente con síndrome antifosfolípido y pérdida gestacional recurrente / Use of low molecular weight heparin and aspirin in a patient with antiphospholipidsyndrome and recurrent pregnancy loss

Introducción: La pérdida gestacional recurrente (PGR) es una patología frecuente, que causa gran impacto en las parejas conllevando frustración, ansiedad, depresión, gastos excesivos y hasta rupturas de la relación de pareja. Su etiología es multicausal y frecuentemente no se logra determinar . Se presenta el caso de una paciente con PGR quien luego de seis pérdidas gestacionales, en el séptimo embarazo se detectaron anticuerpos antifosfolípidos altos, recibiendo tratamiento de tromboprofilaxis doble hasta la semana 32, obteniéndose un producto pretérmino vivo y sano que evolucionó satisfactoriamente. Se discuten dificultades para la precisión diagnóstica y las modalidades de tratamiento descritos. [Salazar HA, Lancheros EA, Becerra CH. Uso de heparina de bajo peso molecular y aspirina en una paciente con síndrome antifosfolípido y pér-dida gestacional recurrente. MedUNAB 201 1;14(3):188-192].

Recurrent pregnancy lost (RPL) is a common condition that causes great impact on partners leading frustration, anxiety , depression, excessive spending and even breaks in the relationship. Its etiology is multifactorial and often can not be determined. This paper describes a case of a patient who after six RPL losses, in the seventh pregnancy is detected high positive antiphospholipid antibodies. She receive thromboprophylaxis twice a week until 32 weeks of gestational age, produced an healthy preterm newborn with a good prognosis. We discuss difficulties in the diagnostic accuracy and treatment modalities described. [Salazar HA, Lancheros EA, Becerra CH. Use of low molecular weight heparin and aspirin in a patient with antiphospholipidsyndrome and recurrent pregnancy loss. MedUNAB 2011;14(3):188-192].

The climical value of serum progesterone and estradiol to the diagnosis of embryo arrest in early pregnancy patients / 中国基层医药

Objective To analyze the clinical relationship of progesterone(P) and estradiol(E2) for embryo arrest in early pregnant patients.Methods 118 patients with embryo arrest in early pregnancy were selected and divided into two groups,research group (58 cases,embryo arrest in early pregnancy) and control group (60 cases,threatened abortion but embryo keeping growth by tocolysis).Serum P and E2 were measured and compared between two groups.Receiver operating characteristic curves(ROC) of all indexes were drawn and the areas under the ROC curves were calculated to evaluate diagnostic value.Results Compared to control group,the serum P and E2 were lower in research group(P ＜ 0.05).To diagnosis embryo arrest,the threshold for P was less than 118.4nmol/L,with 93.9％ sensitivity and 87.3％ specificity; while the threshold value for E2 was less than 1110pmol/L,with 95.6％ sensitivity and 90.7％ specificity.Conclusion Detection of serum P and E2 may be helpful for the diagnosis of embryo arrest in early pregnancy,and the serum E2 had the best diagnostic value.